What is Botulinum Toxin Type A??
Botulinum Toxin Type A (BoNT-A, CAS 93384-43-1) is a 150 kDa zinc-dependent metalloprotease produced by Clostridium botulinum. It consists of a 100 kDa heavy chain (receptor binding and membrane translocation) disulfide-linked to a 50 kDa light chain (catalytic endopeptidase). BoNT-A cleaves SNAP-25 (synaptosomal-associated protein 25 kDa) at the presynaptic neuromuscular junction – preventing SNARE complex assembly required for acetylcholine (ACh) vesicle fusion and release. This produces localised chemodenervation lasting 3-6 months until new nerve terminal sprouting restores function. BoNT-A is both the world’s most potent biological toxin and one of the most widely used therapeutics, forming the mechanistic basis for cosmetic wrinkle reduction, spasticity treatment, hyperhidrosis, migraine prophylaxis, and autonomic disorder research.
Research Applications
SNARE Complex & Neuromuscular Biology
SNAP-25 endoproteolysis, SNARE complex inhibition kinetics, ACh vesicle exocytosis blockade mechanisms
Chemodenervation Research
Motor end-plate remodelling, nerve terminal sprouting/recovery, NMJ plasticity timecourse studies
Cosmetic & Dermatology Research
Glabellar/forehead wrinkle reduction mechanisms, mimetic muscle paralysis, collagen remodelling under denervation
Spasticity & Movement Disorders
Upper motor neuron syndrome models, dystonia, cerebral palsy – focal muscle hyperactivity quantification
Autonomic & Pain Research
Hyperhidrosis sweat gland denervation, chronic migraine CGRP pathway interaction, autonomic ganglia studies
Quick Specs
| Form | Lyophilized powder |
| Purity | >=99% by HPLC – every batch |
| CAS Number | 93384-43-1 |
| Molecular Weight | MS molecular weight confirmed – every batch |
| Specification | >=99% by HPLC – every batch |
| Shelf Life | 24 months unopened |
| Storage | -20C sealed | Do NOT reconstitute until use |
| Manufacture | In-house Fmoc SPPS – Qingdao, China |
| Brand | Asia |